Does Ins-1 cells survive well in hypoxic conditions? If not, what alternative neuroendocrine cancer cell line can be used for experiments in hypoxia?

12 December 2017 1 7K Report

Ins-1 is adherent pancreas cells from rat.

Hi Sutonuka, so far as I know, it is quite difficult for cancer cells survive for a long time under hypoxia, because in vitro hypoxic condition (1% oxygen) cannot fully mimic in vivo physiological hypoxia condition. Therefore you need to design and finish your experiments within a limited incubation time.

If I were you, I'll culture Ins-1 cells under hypoxia, and evaluate cell viability at different time points. It depends on cancer types and cell context.

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