Sutonuka Bhar

6 Questions 3 Answers 0 Followers

Questions related from Sutonuka Bhar

Which software is the most appropriate for first time users for doing scientific drawings (for publication)?

I want a software advance than paint but less complicated than photoshop for creating diagrams for my scientific posters and publications. Kindly help.

08 August 2018 1,007 25 View

Which is the best antibody for chromogranin B?

Over past few months I have tried CgB antibodies from different manufacturers and they either do not detect well, or show a lot of background. I was previously using Chr-b (C 19) from Santa Cruz...

08 August 2018 4,647 2 View

Which lipid is most compatible with size exclusion chromatography using G-25 resin?

I am reconstituting purified proteins in lipid vesicles and then doing gel filtration with G-25 resin to obtain vesicles of only a particular size. However, I found some lipids do not elute out of...

07 July 2018 8,263 6 View

Do Sf9 cells have chromogranin B already present?

I infected Sf9 insect cells with baculovirus containing chromogranin A construct, in different concentrations and did western blot for detection of overexpression of chromogranin A. I found...

05 May 2018 1,847 0 View

Does Ins-1 cells survive well in hypoxic conditions? If not, what alternative neuroendocrine cancer cell line can be used for experiments in hypoxia?

Ins-1 is adherent pancreas cells from rat.

12 December 2017 6,595 1 View

Will Cobalt Chloride precipitate in presence of FBS in cell culture media?

I am planning to use cobalt chloride hexahydrate to incude HIF-1a in my cells which grows in RPMI+FBS media. Will cobalt chloride cause precipitation of proteins in media during incubation?

12 December 2017 1,334 2 View