I infected Sf9 insect cells with baculovirus containing chromogranin A construct, in different concentrations and did western blot for detection of overexpression of chromogranin A. I found single dark bands in both infected and uninfected cells.
I want a software advance than paint but less complicated than photoshop for creating diagrams for my scientific posters and publications. Kindly help.
07 August 2018 1,032 25 View
Over past few months I have tried CgB antibodies from different manufacturers and they either do not detect well, or show a lot of background. I was previously using Chr-b (C 19) from Santa Cruz...
07 August 2018 4,668 2 View
I am reconstituting purified proteins in lipid vesicles and then doing gel filtration with G-25 resin to obtain vesicles of only a particular size. However, I found some lipids do not elute out of...
06 July 2018 8,292 6 View
I am planning to use cobalt chloride hexahydrate to incude HIF-1a in my cells which grows in RPMI+FBS media. Will cobalt chloride cause precipitation of proteins in media during incubation?
11 December 2017 1,361 2 View
Ins-1 is adherent pancreas cells from rat.
11 December 2017 6,626 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Excavation in 2007 in Northern Scotland along the East Coast revealed a domestic site containing the unbroken half (other half is missing) of a perforated circular disc measuring L 37mm x W...
27 July 2024 6,019 0 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
I need help for find a subject for PhD in insect physiology
12 July 2024 8,227 1 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
I want to know what are the relationship that happen between insect biodiversity and durian tree
10 July 2024 8,230 1 View