I infected Sf9 insect cells with baculovirus containing chromogranin A construct, in different concentrations and did western blot for detection of overexpression of chromogranin A. I found single dark bands in both infected and uninfected cells.
I want a software advance than paint but less complicated than photoshop for creating diagrams for my scientific posters and publications. Kindly help.
07 August 2018 967 25 View
Over past few months I have tried CgB antibodies from different manufacturers and they either do not detect well, or show a lot of background. I was previously using Chr-b (C 19) from Santa Cruz...
07 August 2018 4,597 2 View
I am reconstituting purified proteins in lipid vesicles and then doing gel filtration with G-25 resin to obtain vesicles of only a particular size. However, I found some lipids do not elute out of...
06 July 2018 8,219 6 View
I am planning to use cobalt chloride hexahydrate to incude HIF-1a in my cells which grows in RPMI+FBS media. Will cobalt chloride cause precipitation of proteins in media during incubation?
11 December 2017 1,286 2 View
Ins-1 is adherent pancreas cells from rat.
11 December 2017 6,541 1 View
Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...
01 March 2021 7,168 9 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
23 February 2021 8,609 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
This insect is minute in size around 1 mm and not clearly visible with naked eye.
22 February 2021 5,907 11 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...
22 February 2021 1,777 3 View