What is the relation between Zero Point State and Vacuum energy?

Is the vacuum energy, the energy of the Zero Point state in Quantum Mechanics?

03 June 2020 8,934 2 View

Is it possible to use the same figure in two articles?

I have done an experiment and I want to write two articles about it. It has a main data and figure ( The optimum dose and time). Is it possible to use the main figure in two different articles?...

21 April 2020 927 11 View

Whats your idea about Au mineral and Bi mineral in Quartz veins ?

I saw two minerals in quartz veins (Au mineral and Bi mineral ) in an epithermal deposit. could you please help me how to interpret it.

04 February 2020 1,307 4 View

How to name the ore minerals by element?

I have a mineral with 72 percent Bi.

27 December 2019 3,761 3 View

What is the general equation of heat transfer in solidification?

beside equations of heat transfer with mechanisms of Conduction, Convention and Radiation, is there a general equation to calculate the total heat transfer?

11 October 2019 876 3 View

Please explain this Correlation diagram?

Hi Would you mind please explain this correlation diagram between gene Y and X expression. What is R Sq Linear? What is rs? Thank you

17 June 2019 3,848 5 View

Will DEPC-treated water after autovlave negatively affect on RNA-sequencin?

i want to wash eppendorf tubes and laboratorial dishes with DEPC-treated water (incubate for 3 hours) and autoclave them in 120 c for RNA extraction and RNA sequencing. is dose DEPC interfer...

10 January 2019 3,111 3 View

SiRNA Transfection: effects of Time and Dose?

I have a question about transfection method with siRNA and transfection reagent. How can we explain the best transfection dose with 60 pmol among different doses (20, 40, 60, 80)? Why did not 80...

08 December 2018 858 4 View

Can we use controllers of other articles in ours?

Hi I have a question.We did a clinical research for measuring a specific CD marker in special patients. But we forgot to measure it in normal people. Can we use the measurements of this marker as...

19 August 2018 1,406 3 View

Does freezing effect DMSO (For MTT) efficiency?

I found that DMSO that is used for MTT assay is freezed just for once by a person mistake? Does it effect DMSO (For MTT) efficiency?

12 June 2018 7,416 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View