Hi
Do you know that siRNA can be inserted into the white blood cells of normal people and leukemic people sample?
It's theoretically possible, but they don't allow you to just treat people experimentally. If you have the cells in culture, you can put the siRNA in a liposomal carrier and they will take it up.
I am trying to do Roughness Measurement for Alumina templates . I am getting streaks when playing around with I-Gain & P-Gain but not a good image of publication quality. Could someone suggest...
30 May 2024 2,956 4 View
we work on forward osmosis membrane. the support layer is aminated PVC and the active layer is polyamide. for synthesis of polyamide , I follow the routine methods of interfacial polymerization...
19 April 2024 3,574 1 View
Hello. Can you introduce a traditional alloy or HEA, with high temperature (1200 degree) Oxidation resistance and boiling HNo3 and HCl resistance?
13 November 2023 8,479 1 View
Dear friends How to detect Asparaginase resistant cell lines? Do you know cell lines resistant to asparaginase? Is there any protocol to detect the resistance?
20 August 2023 7,261 1 View
Dear colleagues Please suggest a comprehensive and complete protocol for the production and purification of bacterial recombinant protein in E.Coli BL21 strain.
10 July 2023 8,318 2 View
for example, how can I calculate c-index or c-statistic (for investigation of my prediction model performance) at 5-year and 10-year follow-up in cohort sample?
04 October 2022 6,666 2 View
I want to make scfv from E.coli, but I can't find a standard and clear protocol. Please help me if you can🙏
20 September 2022 2,378 1 View
What type of phosphate buffer is suitable for extracting protein from the plant I intend to extract and measure protein from plants by using the Bradford method. In order to do this, I need a...
13 March 2022 2,771 6 View
Hello. My XRD data shows that with prolonging the milling time, peaks become more sharp and have higher intensity. Is there an explanation for it?
08 February 2022 743 7 View
Dear all, I am working on a string matching project. I want to find the best and most appropriate index in each array of the string to divide it into two groups so that it has the lowest cost if a...
02 February 2022 9,481 9 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View