14 Questions 13 Answers 0 Followers
Questions related from Masoumeh Fardi
Dear friends How to detect Asparaginase resistant cell lines? Do you know cell lines resistant to asparaginase? Is there any protocol to detect the resistance?
21 August 2023 7,296 1 View
Hi Do you know that siRNA can be inserted into the white blood cells of normal people and leukemic people sample?
12 July 2023 7,607 1 View
Dear colleagues Please suggest a comprehensive and complete protocol for the production and purification of bacterial recombinant protein in E.Coli BL21 strain.
11 July 2023 8,347 2 View
I want to make scfv from E.coli, but I can't find a standard and clear protocol. Please help me if you can🙏
21 September 2022 2,413 1 View
Hi Would you mind please explain the difference between assessing viability using Cell Counting Kit-8 (CCK-8) and MTT assay? Thank you
22 October 2020 9,826 3 View
I have done an experiment and I want to write two articles about it. It has a main data and figure ( The optimum dose and time). Is it possible to use the main figure in two different articles?...
22 April 2020 1,137 11 View
Hi Would you mind please explain this correlation diagram between gene Y and X expression. What is R Sq Linear? What is rs? Thank you
18 June 2019 4,042 5 View
Hi Do you know a data base for finding the expression of a desirable gene mRNA in normal people? Thank You
03 June 2019 7,736 2 View
Hi everyone, I have knocked down a specific gene in HL-60 cell line. Then I've measured P53 mRNA expression level. I have noticed that P53 expression was elevated. On the other hand I've read in...
14 May 2019 2,238 5 View
How do EMT inducing transcription factors like SNAIL1 ,ZEB1 and ZEB2 up-regulate many genes expression? I know that for example ZEB1 and ZEB2 dimerizes with E-box motif in different promoters...
19 January 2019 5,133 4 View
I have a question about transfection method with siRNA and transfection reagent. How can we explain the best transfection dose with 60 pmol among different doses (20, 40, 60, 80)? Why did not 80...
09 December 2018 1,049 4 View
Hi I have a question.We did a clinical research for measuring a specific CD marker in special patients. But we forgot to measure it in normal people. Can we use the measurements of this marker as...
20 August 2018 1,601 3 View
Can NBT asses it?
16 June 2018 5,230 1 View
I found that DMSO that is used for MTT assay is freezed just for once by a person mistake? Does it effect DMSO (For MTT) efficiency?
13 June 2018 7,629 5 View
