Masoumeh Fardi

11 Questions 10 Answers 0 Followers

Questions related from Masoumeh Fardi

How to detect Asparaginase resistant cell lines?

Dear friends How to detect Asparaginase resistant cell lines? Do you know cell lines resistant to asparaginase? Is there any protocol to detect the resistance?

21 August 2023 7,194 1 View

Can siRNA be delivered into human normal/leukemia cell?

Hi Do you know that siRNA can be inserted into the white blood cells of normal people and leukemic people sample?

12 July 2023 7,556 1 View

Recombinant protein protocol?

Dear colleagues Please suggest a comprehensive and complete protocol for the production and purification of bacterial recombinant protein in E.Coli BL21 strain.

11 July 2023 8,280 2 View

Do you have a standard scfv production protocol?

I want to make scfv from E.coli, but I can't find a standard and clear protocol. Please help me if you can🙏

21 September 2022 2,348 1 View

What is the difference between assessing viability using Cell Counting Kit-8 (CCK-8) and MTT assay?

Hi Would you mind please explain the difference between assessing viability using Cell Counting Kit-8 (CCK-8) and MTT assay? Thank you

22 October 2020 9,749 3 View

Is it possible to use the same figure in two articles?

I have done an experiment and I want to write two articles about it. It has a main data and figure ( The optimum dose and time). Is it possible to use the main figure in two different articles?...

22 April 2020 1,051 11 View

Please explain this Correlation diagram?

Hi Would you mind please explain this correlation diagram between gene Y and X expression. What is R Sq Linear? What is rs? Thank you

18 June 2019 3,971 5 View

HL-60 cell line and P53 protein??

Hi everyone, I have knocked down a specific gene in HL-60 cell line. Then I've measured P53 mRNA expression level. I have noticed that P53 expression was elevated. On the other hand I've read in...

14 May 2019 2,182 5 View

SiRNA Transfection: effects of Time and Dose?

I have a question about transfection method with siRNA and transfection reagent. How can we explain the best transfection dose with 60 pmol among different doses (20, 40, 60, 80)? Why did not 80...

09 December 2018 976 4 View

Can we use controllers of other articles in ours?

Hi I have a question.We did a clinical research for measuring a specific CD marker in special patients. But we forgot to measure it in normal people. Can we use the measurements of this marker as...

20 August 2018 1,517 3 View

Does freezing effect DMSO (For MTT) efficiency?

I found that DMSO that is used for MTT assay is freezed just for once by a person mistake? Does it effect DMSO (For MTT) efficiency?

13 June 2018 7,531 5 View