What type of phosphate buffer is suitable for extracting protein from the plant
I intend to extract and measure protein from plants by using the Bradford method. In order to do this, I need a phosphate buffer.
Can anyone tell me what kind of phosphate buffer and pH level I should use?
Thank you in advance!
https://alfresco-static-files.s3.amazonaws.com/alfresco_images/pharma/2014/08/22/48b3f375-6c47-4fb0-99e3-a53b21901690/article-87987.pdf
Dear Masoumeh Sadeghinik . See the useful link: https://info.gbiosciences.com/blog/different-types-of-extraction-buffers-and-when-to-use-them
Kindly see also the following useful link: http://oscar.iitb.ac.in/onsiteDocumentsDirectory/Extraction%20of%20Plant%20Protein/Extraction%20of%20Plant%20Protein/Contents/print.pdf
Also check please the following useful RG link: Article A comparative method for protein extraction and 2-D gel elec...
The following link is also very useful: https://www.eurekaselect.com/node/154392/article/protein-extraction-protocol-from-musa-sp-shoots-and-roots-tissue-for-non-reducing-one-dimensional-sds-page-analysis
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts