Biomaterial Question
Hi. I have been trying to synthesising gelatin microparticles using double emulsion method. After adding the oil-gelatin mixture in the ethanol with stirring, the particle seemed to formed but when I washed it with acetone and centrifuge, the precipitates seemed to formed big ball and when I tear it apart it seems like gelatin fiber (instead of microparticle).. Just curious does high speed centrifugation affects microparticle formation? Thanks 🙏