How many mice are needed for long term potentiation?

As above, we are trying to do the Long-term potentiation test using mice hippocampal slices. How many mice are needed or how many slices are needed for LTP? Can anyone help me with that?

15 February 2020 7,073 3 View

Does anyone happen to know how to deal with kernel random matrix without using any expansion (i.e., Taylor, series, orthogonal or bi-orthogonal)?

The literature all depends on some kind of expansion. It is useful for global analysis but not for local.

28 January 2019 6,589 1 View

ARIMA, R square and stationary R square?

Dear all When ARIMA model was used to make the unstable time series data stable. There are many model to choose. which is the best one? Not only BIC value was shown, but also R square,...

24 December 2018 7,782 3 View

Aberrant protein after crispr/cas9?

Hi All, I used Crispr/cas9 to knockout CISH in T cells. The T7EI assay showed the gene editing. However, when I used the Western blot to check the protein expression, I found the the bands with...

12 November 2018 3,755 4 View

Is there any textbook or technical instructions on the preparation of ceramic powder and sample?

Dear all, I am to do some experiment on ceramic powders, alumina for example and wondering how to prepare the powder and sample. I wish to have some recommendations on textbooks and technical...

04 March 2018 4,077 4 View

How to stain RNA for EM?

Does any one know a good reagent (or protocol) to stain nucleic acids (RNA) for electron microscopy (either negative stain or positive stain)? p.s. This is for single particle analysis. Thanks!

31 October 2017 3,663 3 View

HOW TO DO ATRP REACTIONS ON THE SURFACE OF SiO2 NANOPARTICLES?

I encounter two questions during SI-ATRP. My experiment route is shown as follows. First ATRP reaction, MSN-Br(100mg), GMA( Glycidyl methacrylate, 1.5mmol),...

23 December 2016 3,066 3 View

Are deep neural networks practical to problems with low-dimensional input data(e.g. under 10 dimensions) ?

Deep neural network has been proved that efficient in imaging, text and voice data. Are they work good in processing low dimensional data ? (One example, using the DNN to analysis mechanical...

19 May 2016 3,367 3 View

How to do protein purification by intein tag/chitin beads in mammalian cells?

17 April 2016 1,304 2 View

What's the difference between the polarization direction, maximum radiation direction and direction of the electric field distribution?

10 February 2016 7,955 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View