Haiyao Ding

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Questions related from Haiyao Ding

How to prevent tissue falling off from heat-induced antigen retrieval (HIER)?

Hi guys! Currently I am working with HIER on tumor tissue. I used 95dC 20m for HIER but the tissue kept falling off / folding.. I am suspecting that this is because of high temperature. Is there a...

25 November 2024 8,144 5 View

Why cells were stained both for PI and Calcein AM?

Hi! I was staining my tissue with both PI and Calcein AM dyes, but I realize a lot of cells were stained by both PI (red) and Calcein AM (green), for example, the cell at the center of the...

06 September 2024 697 1 View

How to make sure we get regions of tumor cells for spatial transcriptomics in tissue?

Hi guys I am facing an issue regarding spatial transcriptomics. Let's say I am preparing a PPFE for a patient sample. I am only interested in the cancer cells in the tissue. Just from the eye, I...

12 August 2024 9,767 3 View

Does centrifugation in the washing step affect gelatin microparticle formation?

Biomaterial Question Hi. I have been trying to synthesising gelatin microparticles using double emulsion method. After adding the oil-gelatin mixture in the ethanol with stirring, the particle...

07 September 2023 3,953 2 View