Does anyone used the NADP/NADPH quantitation colorimetric kit and can suggest a good positive control?

Hadas Pahima @Hadas-Pahima

08 July 2015 1 3K Report

Hey everyone,

I'm trying to measure the NADPH/NADP levels in A431 cells.

I used 2-DG as a positive control, I expected to see a decrease in this ratio

but I got the opposite result.

Does anyone have tried to do something similar? or have an idea for a better positive control?

Thanks

Caroline Waters

What wave length are you using ? Is this a Sigma Kit ? 450nm is the recommended for colorimetric assay

As far as 2 DG cells are concerned check out 'Beyond Warburg effect - dual metabolic nature of cancer cells' by by J Xie - Nature May ‎2014

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