Hi, I have been analyzing tot glutathione peroxidase (GPx) activity in mussel cells using cumene hydroperoxide (microplates) and I am a bit unsure how to read/calculate the results.
I run a blank (only GPx buffer), cumene test (homogenization buffer+GPx working solution containing GSH and NADPH+cumene) and my samples (samples+GPx working solution+cumene).
My protocol says to correct the values from the background: so does it mean to detract the values from the blank or the ones of the cumene test?
Moreover, it says that background corrected values should not exceed -0.022 OD/min for cumene hydroperoxide, is there any reference where I can see where this value is from?
thank you in advance for the help!