I use Bio-Rad's SYBR Green Master Mix for my qPCR work which, according to the manufacturer's instructions calls for a 50uL reaction volume. I use 20uL on a regular basis without any problems. As I am running a lot of qPCR in the future and looking for ways to save some money, I was wondering if it's realistically possible to use a 10uL reaction volume and achieve the same results in terms of well-to-well variability. My main concern is the inevitable increase in pipet ting error as the reaction volume decreases.
So, does anyone use 10uL qPCR reaction volumes? Have you ever had any issues?