Cody Boyle

10 Questions 11 Answers 0 Followers

Questions related from Cody Boyle

How can I get axon binary files from a windows 10 computer to be read by Minianalysis?

I am recording action potential firing using Clampex software on a Windows 10. These files are opening using Clampfit 10.4 to be analyzed using Minianalysis. Unfortunately, I can no longer analyze...

01 January 2020 8,885 2 View

In a general sense, what are various ways to improve the quality of ELISA experiments and their subsequent data?

Hello, I am planning to run a series of ELISA experiments and am asking for basic tips for improving the quality and reproducibility of my experiments. I have only done the experiment once, but...

02 February 2019 4,722 2 View

How do I determine a proper loading control for my western blot assay using whole tissue homogenates?

I am running a western blot assay using whole tissue homogenates. The homogenates are from collected mouse brains and I am using two groups an Ames dwarf mouse and a wild-type mouse. I see that...

10 October 2018 1,535 3 View

Has anyone gotten a clean doublet when using the Abcam anti-ferritin heavy chain antibody ab65080?

I am using the antibody for western blot analysis of brain tissue homogenates on a 12% gel. Attached is the image of the blot. GAPDH is used as a loading control and is shown around 35 kDa. The...

10 October 2018 2,236 0 View

Has anyone used commercial primers for drosophila e-cadherin? Can I use human?

I have been working through a ChIP-qPCR experiment and am hoping to run a comparative Ct qPCR analysis. I want to use e-cadherin as a housekeeping gene, but realized the primers from my lab are...

07 July 2018 2,474 1 View

When running ChIP assays can you store samples after incubation with Ab and washes for indiscriminate binding?

Can you store immuno-protein complexes prior to DNA isolation or does this experiment need to be run to completion? I only ask because for my protocol there are several incubation periods of 2-4...

05 May 2018 6,894 4 View

QPCR: When analyzing my result, the amplification plots of my samples is a jagged line. What could explain this?

I am running qPCR with gDNA. Using primers tested in a previous experiment, I am not seeing any exponential amplification. In fact, most samples (unknowns and my standards) return with an...

04 April 2018 9,451 8 View

How can one verify pipetting volume of a pipetman?

While running qPCR and using small volumes, I want to ascertain that I am pipetting accurate volumes. I have observed some large variation in my sample quantities.

04 April 2018 1,852 3 View

When running qPCR, what is an acceptable SD for your Ct values?

I have had a variable Standard Deviation of my Ct values for my last few PCR reads. Some SD ~5 and some nearing 10. I am wondering what is considered a large SD and what is acceptable

04 April 2018 5,302 3 View

When writing a manuscript, where should citations go?

I often see them in the middle of the sentence and sometimes at the end, as a collective. I am wondering is there a rule for this type of citation. Is it personal preference or journal specific?

04 April 2018 7,898 8 View