Edward Koellhoffer

5 Questions 11 Answers 0 Followers

Questions related from Edward Koellhoffer

Opinion question: What companies make the best, most reliable Luminex multiplex kits?

This is a question asking for your opinion. I'm wondering which companies and vendors do you think make the best, most reliable Luminex kits available? Or on the other hand, which companies you...

07 July 2018 3,593 2 View

Do you need to statistically correct for the number of analytes examined in a Luminex multiplex cytokine assay?

With Luminex multiplex data, there can be a large number of analytes assessed in a single assay or across multiple assays. In regards to the statistical analysis of the data. I have seen authors...

07 July 2018 4,672 3 View

When transducing primary murine microglia with lentivirus, do you select with puromycin?

I'm looking to transduce primary microglia with lentivirus carrying an shRNA vector that has a puromycin selection component on the vector. However, I see that the methods of some papers where...

01 January 2016 7,162 5 View

What's the best method to process human fetal brain tissue for microglia culture?

I've been trying to culture human fetal microglia from whole human fetal brain samples. I've been using the protocol by Durafort, et al. (link attached). Of course the first time I tried the...

05 May 2015 3,833 4 View

Does anyone run 10uL qPCR reactions?

I use Bio-Rad's SYBR Green Master Mix for my qPCR work which, according to the manufacturer's instructions calls for a 50uL reaction volume. I use 20uL on a regular basis without any problems. As...

10 October 2014 7,337 20 View