7 Questions 15 Answers 0 Followers
Questions related from Sk. Imran Ali
Hi, a quick question? I just shifted to 10ul from our regular 25ul PCR protocol. I used SYBR Green in BioRad CFX. During set up the program, I forgot change the reaction volume from 25 to 10? I...
10 October 2019 3,015 3 View
I want to measure the flouroscent signal from Hoechst stained fixed cell by a plate reader. Do I have to read the plate with PBS in it? Or, I can dry the plate and then can measure it?
10 October 2018 3,587 0 View
I am planning to measure DNA concentration of fixed mammalian cells on 6 well plates. I am using Hoescht for staining. Do I need to use any cell permeabiling agent before I stain them? Should I...
10 October 2018 4,116 3 View
I'm treating my CHO cells with 100uM H202 for positive control. cells are dissociated by 20mM EDTA in 1X PBS. Trying to do all procedure under low light. 1% Low melting agarose temperature was...
03 March 2018 4,325 14 View
About confusing term: Intron, exon and nonfunctional or non-coding regions of genome. Are introns and the nonfunctional regions are same?
09 September 2012 5,765 15 View
Hi, I'm very new in this field. Can anybody tell me where should I start..??? Anybody pls suggest me some paper, from where I can understand what types of work is going on in this field..... I...
11 November 2011 721 17 View
Hello everybody, I'm a new member of this group. I've recently come to known about the term 'Cassette PCR'. But I couldn't gather enough information about it. Can anybody help me...???
06 June 2011 8,157 2 View
