We are trying to find an RPE cell line that expresses bestrophin-1 protein. We have established that hTERT-RPE1 and ARPE-19 express the RNA however do not appear to produce the protein. We would also like to know whether the hTERT-RPE1 cells can become fully differentiated?
ARPE-19 cells express Bestrophin when cultured to longer period i.e. when they are fully confluent. The expression of Bestrophin also depends on which substrate the ARPE-19 cells are growing. At least in our case, ARPE-19 cells whole protein extract from fully confluent culture show Bestrophin expression in WB. The cells should be left till one more week after they attain confluence. Polarization of cells is also achieved thorough culturing them on Bruch's membrane till confluence. In case of hTERT-RPE1, I do not have experience.
Hi
Could you help me? I know that this is not the answer to Kristen. However I want to ask you something since you employ arpe-19.
I have noticed that growing these cells in Dmem/F12 + 10% SFB change their morphology and start to appear a round population like stem cells. Now O am trying to feed them only with dmem + 10% SFB... do you think that these cells can expand in this type of medium? What I can do?
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