Best primers for human genes COXI and COXIV?

04 June 2024 1 7K Report

Hello everyone! My PI would like me to measure the amount of mitochondrial DNA in multiple cell lines using qPCR targeting COXI and COXIV. However, I have never done a qPCR that measures mitochondrial genes. Does anyone have any primer recommendations for these genes?

Also, should I use cDNA or gDNA for this experiment?

Katie A S Burnette

Since you are wanting to measure mitochondrial genome copy number, you want a DNA extraction that includes mtDNA.

cDNA is made from mRNA, which will give you transcript abundance, not genome copy number.

Start by reading the published literature & also the MIQE guidelines.

Good luck!

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