How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 4,866 2 View

Why are my SYBR green standard curve dilutions not amplifying and have flat curves?

Please help! Recently our SYBR Green qPCR reaction using our housekeeping gene, RPLP0, and all the same conditions stopped working one day to the next. We isolate RNA from tissue culture human...

03 August 2017 2,418 13 View

How to extract enough RNA from jellyfish polyps (Aurelia aurita) to monitor the expression variations of some genes (RT-PCR and qPCR)?

I would be very grateful if the community could give me some tips: as these organisms are very small, I wonder how many polyps do I have to pool for one RNA extraction or if one is enough? What is...

29 January 2017 4,605 2 View

In case of infected root samples, is the infection stopped by the Gus solution or by the ethanol washing bath (+ ref please :) )?

In my case, I'm working with entire root systems of Medicago truncatula infected by an oomycete.

18 October 2016 2,471 4 View

Why can I not digest or sequence my plasmid?

I amplified a plasmid and now I have an eluted stock from a maxi prep i did. I sent the plasmid out for sequencing and it came back as no priming. I tried a restriction digestion with 2 different...

13 August 2015 4,170 6 View

Any suggestion on the seeding density for primary monocytes in a 96-well?

I am doing a one-day assay with primary monocytes and seeding them at 3*10^5/well, as per a colleague recommendation. However I see others recommend much lower, but they seem to have different...

03 June 2015 6,422 2 View

How do I stimulate cell cultures with beta-estradiol?

I have been doing cell culture experiments that involve treating the cells with estradiol (E2). There are many papers published that state these cells are responsive to estrogen. The problem is...

15 May 2015 2,953 6 View

Can I use DataAssist or ExpressionSuite software for analysis if I acquired my qPCR data on the LightCycler 480?

As opposed to using Excel for analysis.

23 January 2015 5,201 1 View

Are you interested in collaborating on work on residential property markets in Australia and China?

I am looking for academics interested in residential property markets in Australia and China. Thanks Justine

29 October 2014 5,524 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View