How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

Has anyone seen this perivascular black stuff in rat brains?

This photo is from a reportedly healthy Long-Evans rat. Several of these animals have similar findings. No other sign of illness. Has anyone out there seen this?

19 November 2020 8,108 4 View

Working with nominal data in Excel / Stat plus, Chi square?

We are analyzing the response of >400 respondents to a survey, with values of -1 (no) 0 (neutral), and +1 (yes). The data were collected to assess opinions on feeding practices at zoos. An...

17 October 2020 953 2 View

What are the best approaches for manipulating gene expression in specific cell types of birds in vivo?

I am a PhD student currently working with zebra finch. I think that the ability to manipulate the gene expression of specific cells within living zebra finch embryos would be useful for my...

19 January 2019 4,101 1 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 4,866 2 View

When making SDS-PAGE gels I have bubble formation between the gel and plastic comb. Typically on the end of the gel. Is there a way to prevent this?

I usually lose one or two lanes as a result of the bubbling. Is this typical? Please see photos. I have no visible issues of leaking. I use fresh APS. I clean my plates rigorously. Thanks to...

24 April 2018 9,625 8 View

Why are my SYBR green standard curve dilutions not amplifying and have flat curves?

Please help! Recently our SYBR Green qPCR reaction using our housekeeping gene, RPLP0, and all the same conditions stopped working one day to the next. We isolate RNA from tissue culture human...

03 August 2017 2,418 13 View

How to extract enough RNA from jellyfish polyps (Aurelia aurita) to monitor the expression variations of some genes (RT-PCR and qPCR)?

I would be very grateful if the community could give me some tips: as these organisms are very small, I wonder how many polyps do I have to pool for one RNA extraction or if one is enough? What is...

29 January 2017 4,605 2 View

Does anyone know what the average cell diameter of Panc1 cells is?

27 November 2016 5,872 3 View

In case of infected root samples, is the infection stopped by the Gus solution or by the ethanol washing bath (+ ref please :) )?

In my case, I'm working with entire root systems of Medicago truncatula infected by an oomycete.

18 October 2016 2,471 4 View

Which are the factors that determine in a tourist the destination for their travel?

I'm working on a study of how selection criteria of Tourism have changed considering Pandemic + Vaccination expectation, so propose a new prediction behavioral dynamic model for Riviera Maya (Mexico).

09 February 2021 314 7 View

What are the environmental variables that influence the Northeastern gray whale population?

I am currently working on my M.Sc. thesis studying how climate change affects the presence of the gray whale in one of its main breeding areas in Mexico. We have considered temperature,...

16 November 2020 8,944 3 View

If an observed d falls beyond the upper limit in equivalence testing, what does that mean?

I have been taking your Coursera Classes and reading some of your articles on equivalence testing, and I do not know how to interpret and effect and its corresponding CI, but I do not how to...

12 October 2020 9,046 5 View

What kind of small mobile robots are available on market to undertake multi-robot research?

Dear All, What kind of small mobile robots are available in market to undertake multi-robot research? Would you have anything to be recommended? Thanks! Chaomin Luo ~~~ Chaomin Luo,...

04 October 2020 5,173 5 View

Is the amino acid mutant D614G of SARS-Cov -II most infectious?

Variants of SARS-CoV-2 with a widespread mutation are more infectious in human cells and hamsters, compared with viral variants lacking the change.In February 2020, researchers examining samples...

01 October 2020 1,886 5 View

Q1. What changes have been observed in the professional lives of our CE graduates ? Q2. What CE academic programs have developed?

In June 1995, the culmination of some 3 years or so of preparation, led by professor Dr. Jim Yao, Texas A&M, resulted in an "ASCE Education Conference" in Denver, CO. At the end of that...

12 August 2020 1,069 3 View

Which railway magazines in South-/Central America and Mexico to publish - who has a recommendation?

Which Railway magazines are relvenat in Middle and South America? We want to publish a technical article about vibration reduction measures carried out in Mexico. Who has a recommendation or give...

18 May 2020 3,487 4 View

How to arrange cross-sectional pooled data in excel..?

I am working on a project which one country has ten trade partner countries I will take ten time period data, for example, US trade with Canada, China, Mexico and Brazil (US export and also...

15 December 2019 8,047 2 View

Can anyone kindly share strong motion data of mexico 1985 and victoria?

I am presently working on a project which requires strong motion data of mexico 1985 and victoria. I tried to search databases but files are either corrupted or removed. kindly if available share...

06 July 2019 2,402 2 View

In need of Experts to Review 2 Data Sources for a Dissertation/Qualitatives Study, Can You plese Read and Consider Participation?

Greetings from Houston, Texas, Preparing my proposal for approval and am in need of three PhD's to review 2 qualitative data sources for the study. Data source 1 is a semi structured interview,...

30 May 2019 6,155 4 View