5,000 um^2 is at the extreme upper range. Generally spread MSCs have a diameter ranging from 30 - 60 microns, so 700-3,000 um^2.
Dear Yanni,
it depends where you look at them. In culture they reach a length up to some hundred micrometers. Factor determinating the MSC size are the density of the cells, the surface they sit on and the Age of the cells. The MSC get bigger and fried egg shaped the older they get. Regarding their size Suspension I agree with the answers above. Based on flowcytometry I roughly estimate the double Diameter of peripheral blood lymphocytes which were around 10-12 µm.
Best regards
Dirk
I thank you all for the good and useful answers. i am working with dental pulp stem cells and I will start to register their average size, general morphology and surface markers. Thank you and all the best for you.
Gustavo
Yanny... you asked this question 2 years ago. did you find any reference for MSC size??
This paper did a really good job at quantifying MSC diameter: http://www.ncbi.nlm.nih.gov/pubmed/24390934
Abstract
BACKGROUND AND PURPOSE:
Intravascular injection of mesenchymal stem cells (MSCs) has been found to cause considerable vascular obstructions which may lead to serious outcomes, particularly after intra-arterial injection. However, the underlying mechanisms have been poorly understood.
METHODS:
In this study, we fractionated MSCs that had been cultured in monolayer for six passages into small (average diameter = 17.9 μm) and large (average diameter 30.4 μm) populations according to their sizes, and examined their vascular obstructions after intra-internal carotid artery injection in rats and mice in comparison with MSCs derived from 3D spheroids which were uniformly smaller in size (average diameter 12.6 μm).
RESULTS:
We found that 3D MSCs did not cause detectable infarct in the brain as evidenced by MRI scan and TTC stain, 2D MSCs in small size caused a microinfarct in one of five animals, which was co-localized to the area of entrapped MSCs (labeled with DiI), while 2D MSCs in large size caused much larger infarcts in all five animals, and substantial amounts of DiI-positive MSCs were found in the infarct. Meanwhile, corresponding neurological defects were observed in the animals with stroke. In consistence, injection of 2D MSCs (average diameter 26.5) caused a marked loss of cortical neurons and their axons in Thy1-GFP transgenic mice and the activation of microglia in CX3CR1-GFP transgenic mice in the area with MSC entrapment.
CONCLUSIONS:
Our results suggest that the size of MSCs is a significant cause of MSC caused vascular obstructions and stroke.
We have data that size of human adipose-tissue mesenchymal stem cells is 50 um2.
You can get the size of MSC in following article: doi: 10.1007/s12015-013-9492-x
If giving intravenous umbilical cord derived mesenchymal stem cells would a 0.2 micron filter block out the MSCs?
Yes,
MSC will be larger than RBC.
In culture when MSCs are attached the size is larger than in suspension. But it will be more than RBCs.
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