Berrin Saracoglu

10 Questions 27 Answers 0 Followers

Questions related from Berrin Saracoglu

Is there any antibiotic residual test for cell cultures?

Hello everyone; I need to show that there is no residue after removing the antibiotic from the cell culture. Is there any test/assay to analyze it? Thank you B.S.

29 January 2022 5,897 4 View

Looking for a mRNA data website, any recommendation ?

Hello, There is a specific mRNA that I want to work with it but I need some data about it such as; amino acid sequence, how many bases, the length or molecular weight..etc? Is there any...

07 July 2021 3,959 3 View

Human Dermal Fibroblast Quality Assays ?

Hello all; I am going to isolate, culture and do quality assays for Human Dermal Fibroblasts (HDFs). I checked the literature review BUT STILL WOULD LIKE TO GET YOUR OPINIONS as this will be my...

31 May 2021 9,487 5 View

Could any one check my Bone Marrow Derived MSC Isolation protocol please ?

Hello; I isolated Mesenchymal Stem Cells (MSCs) from Bone Marrow (BM). My very first 3-4 isolation was all right but then I started to have some round different cells in my culture. Microbial...

09 January 2019 6,162 11 View

Cell recovery from Ficoll or Percoll?

Hello;Is there any way to recover blood that mixed with percoll or ficoll by a mistake?

31 January 2017 5,878 6 View

Natural Killer (NK) cell protocol for clinical applications?

Hello;We want to use NK cell for our hematologic oncology patients. I found some papers about it but couldnt be sure. If you could share your protocol or at least address me to a paper please I...

29 April 2016 6,352 2 View

Could you suggest a Bioreactor for MSCs?

Hello Guys;If any of you could suggest me a bioreactor for bone marrow derived MSC culture I would appreciate it. At least what should be the criteriaons? or any brand? Thank you Berrin

20 November 2015 968 4 View

How do we choose culture medium type; DMEM,MEM,RPMI?

Hello guys;I would like to learn how we can choose medium type. for example some papers say alpha-MEM is better than DMEM for human Mesenchymal Stem Cells (hMSCs) and some says DMEM is better than...

07 January 2015 4,033 25 View

How do B27 and insulin affect mammospheres?

I reached your paper but it is in Chinese so I could not understand it. Could you help me to understand how B27 and insulin affect MCF-7 derived mammospheres please? Thank you

18 March 2014 7,888 1 View

Cholera toxin

I am going to work with MCF-7 derived mammospheres. I need cholera toxin. Most companies sends in within 2-4 months. Any suggestion where I can get it? I am in Italy.

23 July 2013 2,001 2 View