Can anyone suggest what are these large bubble like structure along with small organoids and how to get rid of them?see the attached image.?

I am growing 3D organoids from mouse lung AT2 cells in matrigel. I am using glutamine, Penstrep. 10% FBS, ITS and tGF b inhibitor in media.after 6 days I observe large bubble like structure in the...

03 January 2020 1,430 5 View