I'd like to block Jurkats in g1/S with thymidine and g2/M with nocodazole.
The results are form Likert skill
28 June 2024 7,758 3 View
I am writing a paper on the rights and obligations of corporations that choose to take public positions on social issues. I am very keen to collaborate with others interested in this topic.
27 June 2024 5,924 1 View
Most communities in New Mexico have families lacking access to vital services for surviving and thriving including: medical care, mental healthcare, food security programs, housing security...
21 March 2024 1,767 1 View
According to the research, Red and White Blood Cell Morphology Characterization and Hands-on Time Analysis by the Digital Cell Imaging Analyzer DI-60, held in Chung-Ang University. The capability...
07 February 2024 5,749 2 View
Despite an extensive search, I have not found anything employing a similar striping technique. Current expertise suggests that the origin of the painting may be the town of Adria in the Rovigo...
04 December 2023 3,485 0 View
Pre- and Post Analytical errors in Hematology source: De la Salle, B. (2019). Pre‐ and postanalytical errors in Haematology. International Journal of Laboratory Hematology, 41(S1), 170–176....
03 September 2023 7,979 1 View
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25 June 2023 5,584 0 View
Hi all, I've recently upgraded from the Agilent Seahorse XF24 to the XFe24 and have started having issues with the OCR calibration. The rep suggested that storing the hydrated cartridge at 4C even...
26 January 2023 3,288 3 View
Hi, I'm currently writing my research proposal for my MSc project and I've gotten myself entangled into a dangerous and confusing world of epistemology and ontology. I am aiming to carry out...
26 December 2022 5,042 0 View
I need to have a very high dillution of it 1/3k-1/5k and a very low background on my homemade ELISA. Best I have is 1/1000 but cost a lot because I do a lot. Any suggestion? Thank you
06 December 2022 8,393 2 View
Hello, I am currently exploring different thymidine treatments on Hela Cells. However, my Fascan profiles consistently reveal two peaks, suggesting that none of the thymidine conditions are...
07 April 2024 8,063 1 View
Hi, I am now studying a cell-cycle-dependent protein with HeLa and Hek293. I used double thymidine method for cell cycle arresting. However, I have found that cells (both HeLa and Hek293) seemed...
18 November 2022 3,417 0 View
Hello everyone, I am working with budding yeast. Recently, I came across some type of contamination in my live culture. I added 100X microscope image of my live culture. These string type things...
16 November 2022 6,946 3 View
I'm trying to synchronize MCF-10A cells with double thymidine block to enrich a centrosomal protein at G1/S transition following this protocol: 1) Add thymidine to final 2 mM for 12h (from a 100...
01 March 2022 9,579 1 View
Hi all, I am currently learning both how to perform relatively simple flow cytometry experiments revolving around the use of propidium iodide (PI) as a DNA marker to look at the cell cycle and...
01 February 2020 8,841 3 View
I initially plan to synchronize cells at G2/M using nocodazole (after 22 hours of serum-free media plus a two-hour recovery with complete media). However, I just learnt that it would be better to...
23 December 2019 6,524 0 View
I'm planning to synchronize Hela cells at early S phase using double thymidine block. I've read different protocols, suggesting different time periods for the incubation of cells after the...
19 December 2019 5,664 0 View
After nocodazole treatment for cell cycle synchronization, most of my cells became round, but didn't float. What happened to them? Can I continue to lyse them for coIP and western blot?
11 December 2019 4,907 9 View
I have been applying a double thymidin block to my NIH3T3 cells to synchronize them. The aim is to fix them in mitosis and perform some immunocytochemical stainings. Although I see a good amount...
11 December 2019 6,639 2 View
I am trying to arrest budding yeast cells at 30°C and 42°C. While at 30°C after 1.5 h with 10 ug/ml Nocodazole I observe a population of metaphase-arrested cells, at 42°C I see cells at various...
14 November 2019 1,031 3 View