How do I find out if a Journal is indexed in Scopus, Scimago or Thompson Reuters?

How do I find out if a Journal I want to publish with is indexed in Scopus, Scimago or Thompson Reuters?

10 October 2020 4,515 4 View

What's wrong with my culture of NK92 cells?

Hi everyone, I am trying to culture IL-2 independent NK-92 cell line for weeks now but they are still not proliferating. Media used: MEM with 10% NU serum, 0.2 mM myo-inositol, 0.02 mM folic...

27 September 2020 2,564 6 View

Number of Estimating Equations Required to Test Hypotheses?

After literature review, current draft as below: Variables: Independent = B (represented by I, M, O, S, C), Moderating = W Dependent = F Control = CV Hypotheses: H1a. I has significant...

18 September 2020 7,055 2 View

What is the difference between Introduction and Background in a manuscript?

Some journals do require that a manuscript contain Introduction as a heading as well as background as another heading. This clear separation make it difficult for distinguishing what information...

08 March 2020 7,479 11 View

What is a Case Study and how should I do it?

For my dissertation (PhD) I am looking for something that does not require statistics per se. I am thinking of using a Case Study method. Can I use this for analyzing a group (Christian...

24 February 2020 6,951 4 View

I am looking for a spreadsheet to calculate the XYZ coordinates of a few small batch of samples collectected along deep angled drillholes?

Hello, I am working on stratigraphy and lithogeochemistry of a VMS-hosting sequence of Paleoproterozoic volcanic rocks and would like to plot drillcore samples that I collected into IoGas and...

23 October 2018 5,317 7 View

In fusion proteins, why is the fusion peptide region hydrophobic?

I would like to understand how the hydrophobicity of the region assissts in membrane fusion

25 June 2018 6,796 3 View

How do you treat forced swim test data?

My design is a two factor 3x5 (3 hormone conditions and 5 drug conditions, 15 groups total) between-subjects ANOVA. When rats undergo the 15 minute pre-test habituation forced swim they have not...

11 June 2018 1,558 3 View

Any syllabi about scientific writing for social science students?

I am planning on teaching a class on scientific writing (mostly for social scientists). Any examples of syllabi in this area that you are aware of?

15 April 2018 5,717 6 View

How long do I leave a brain in isopentane when flash freezing it for future Western blot analysis?

I have flash frozen brains in the past using isopentane with dry ice in it but I found that some of them cracked. What is the optimal time to leave the brain in the isopentane such that it is...

03 March 2018 2,998 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View