I need to have a very high dillution of it 1/3k-1/5k and a very low background on my homemade ELISA. Best I have is 1/1000 but cost a lot because I do a lot. Any suggestion?
Thank you
The ratio of A403 to A275 (heme content) may be used for assessing the purity of HRP preparations, it should be >3 for preparing sensitive conjugates.
ty but not always availlable before you buy and do it yourself.
The results are form Likert skill
28 June 2024 7,758 3 View
I am writing a paper on the rights and obligations of corporations that choose to take public positions on social issues. I am very keen to collaborate with others interested in this topic.
27 June 2024 5,924 1 View
Most communities in New Mexico have families lacking access to vital services for surviving and thriving including: medical care, mental healthcare, food security programs, housing security...
21 March 2024 1,767 1 View
According to the research, Red and White Blood Cell Morphology Characterization and Hands-on Time Analysis by the Digital Cell Imaging Analyzer DI-60, held in Chung-Ang University. The capability...
07 February 2024 5,749 2 View
Despite an extensive search, I have not found anything employing a similar striping technique. Current expertise suggests that the origin of the painting may be the town of Adria in the Rovigo...
04 December 2023 3,485 0 View
Pre- and Post Analytical errors in Hematology source: De la Salle, B. (2019). Pre‐ and postanalytical errors in Haematology. International Journal of Laboratory Hematology, 41(S1), 170–176....
03 September 2023 7,979 1 View
non
25 June 2023 5,584 0 View
Does anyone routinely use BODIPY 581/591 C11 to look at lipid peroxidation by flow cytometry? The theory is that unoxidised BODIPY fluoresces with an Em around 581nm but the oxidised form...
15 October 2022 5,728 0 View
It is like Web of Science is merging with other indexing houses. Please share if you have full details of goings on there
17 August 2022 3,522 0 View
I want to know if the structure of an original journal paper (Introduction, Materials and Methods, Result and Discussion & Conclusion), is the same as a reviewed journal paper. If not, what is the...
28 May 2022 9,253 2 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View
I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...
15 June 2024 5,109 1 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View
We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?
05 June 2024 2,173 5 View
I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...
05 June 2024 8,191 0 View
I want to measure a protein in serum using an ELISA kit. This kit allows serum samples to be diluted 200 to 3200 times, but I want to use this kit to titrate higher concentrations of protein. Can...
03 June 2024 4,937 0 View