According to the research, Red and White Blood Cell Morphology Characterization and
Hands-on Time Analysis by the Digital Cell Imaging Analyzer DI-60, held in Chung-Ang University. The capability of DI-60 to recognize specific red blood cell even their indices, and the differentiation of white blood cell was optimal, however, the presence of schizocyte shows that even with high sensitivity, it occurs low specificity, and even abnormal white blood cells with striplings could cause unidentification of the machine. Why cannot the machine recognize the specific morphology and what other limitations can be seen in using the machine even outside of the research.
Overall, the evaluation of the accuracy of RBC morphology characterization and WBC differentials of the Sysmex DI-60 showed acceptable performance in normal samples. However, for abnormal samples, it must be utilized with caution. In RBC morphology characterization, sensitivities for spherocytes and elliptocytes were low, and lower for teardrop cells, thus generating significant false-negative results. Additionally, schistocytes showed sufficiently high sensitivity but significantly low specificity, which could generate false-positive results. As for WBC differentials, the DI-60 showed acceptable performance except for basophils. It was discovered that manual differential counts are still important to report accurate differential counts, especially for basophils, atypical lymphocytes, and normoblasts. Lastly, the DI-60 is not capable of providing the coordinates of a specific cell which will hinder the users to review the cells displayed by the DI-60 through manual microscopy.
The Sysmex DI-60's reliance on automated algorithms may limit its ability to appropriately assess red cell morphology and white cell analysis in the laboratory. While it allows for rapid and high-throughput analysis, it may fail to detect small or infrequent morphological abnormalities in red blood cells (RBCs) and white blood cells (WBCs), thereby affecting diagnostic accuracy. Furthermore, the analyzer may have difficulty distinguishing between certain aberrant cell types, such as nucleated red blood cells (NRBCs) and immature granulocytes, and producing a complete differential white cell count. As a result, while the DI-60 improves efficiency and consistency in regular hematology screening, it may require further manual evaluation by skilled technologists to assure accurate diagnosis and classification of hematopoietic illnesses.
- Badges
- Science method