Dominic Sweeney

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Questions related from Dominic Sweeney

BODIPY 591/581 Lipid Peroxidation Reagent for use in flow?

Does anyone routinely use BODIPY 581/591 C11 to look at lipid peroxidation by flow cytometry? The theory is that unoxidised BODIPY fluoresces with an Em around 581nm but the oxidised form...

16 October 2022 5,728 0 View

HCC70s not growing as quickly as expected?

Hello, would anyone with experience growing HCC70s be able to provide some advice? I was expecting to passage every 2-3 days but the cells don't seem to be growing that quickly when cultured at 5%...

25 May 2022 9,587 0 View

Does anyone know what concentration of thymidine and nocodazole to use for blocking cell cycle progression in Jurkats please?

I'd like to block Jurkats in g1/S with thymidine and g2/M with nocodazole.

09 September 2021 1,462 0 View