Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Can I ask did any good platform or journals for learning the calcluation on the reference governors and command governor?

Recently I face some problems with the mathematical ( calculation) on the reference governor and command governor, so I need some help from you all to get which platform or journal can let me...

23 February 2021 7,746 2 View

How to discriminate between Sentinel-2 Level-2A Product Discriminators?

According to the Sentinel-2 user guide here (https://sentinel.esa.int/web/sentinel/user-guides/sentinel-2-msi/naming-convention), the compact naming convention is arranged as...

21 February 2021 5,919 9 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Flow cytometry and the cell cycle - no mitotic cells?

Hi all, I am currently learning both how to perform relatively simple flow cytometry experiments revolving around the use of propidium iodide (PI) as a DNA marker to look at the cell cycle and...

01 February 2020 8,742 3 View

What happened to my cells after nocodazole treatment?

After nocodazole treatment for cell cycle synchronization, most of my cells became round, but didn't float. What happened to them? Can I continue to lyse them for coIP and western blot?

11 December 2019 4,770 9 View

How to make mitotic NIH3T3 cells sticking to the dish during staining procedure?

I have been applying a double thymidin block to my NIH3T3 cells to synchronize them. The aim is to fix them in mitosis and perform some immunocytochemical stainings. Although I see a good amount...

11 December 2019 6,553 2 View

What is a suitable negative selection marker for introduction a genetic construct into the cancer cell line genome?

I have a question regarding a suitable negative marker for human cancer cell line. Using CRISPR we want to introduce an eGFP reporter construct into a human cancer cell line. But we don't know...

10 November 2019 8,317 1 View

What are the most effective concentration of - Taxol, Nocodazole, SMIFH2, Latrunculin A and Blebbistatin in order to see their affects on cytoskelton?

I'm conducting an expirement in Boyden Chamber to see how different tratments will affect MDA-MB-231 cancer cells ability to migrate. Are there any protocols that can help me decide the specific...

02 September 2019 8,264 1 View

What is the optimum time required for treating transfected HeLa cells with Doxycycline to induce the expression of recombinant GFP tagged protein?

Hi all, I am going to perform immunofluorescence analysis of transfected HeLa cells to determine the localization of the protein of interest.The protein is tagged with GFP and I am using...

05 June 2019 2,230 2 View

What's the difference between [3H]thymidine incorporation and CFSE in cell proliferation experiment?

I am planing to test Treg cells proliferation after activated by TLR2 agonists. I have read some papers and found that some researcher use [3H]thymidine incorporation as a readout, while in the...

08 May 2019 9,086 3 View

What's the difference between [3H]thymidine incorporation and CFSE in cell proliferation experiment?

I am planing to test Treg cells proliferation after activated by TLR2 agonists. I have read some papers and found that some researcher use [3H]thymidine incorporation as a readout, while in the...

04 May 2019 3,614 3 View

What are good methods of synchronizing cancer cells in particularly in G1, S, G2/M stages ?

I am using MCF7 cell line which doubling time is greater than 30 hours, will the thymidine double blocks (block 1; 16 hr-->9 hr release-->block 2: 15 hr ) works better or serum starvation protocol?

04 May 2019 4,127 5 View

Double-Thymidine Syncronization: When Should I perform the Transfection?

28 March 2019 4,259 1 View