I am trying to bind my plasmid DNA to silica columns. 4.5 M Guanidine Thiocyanate, 1 M K acetate PH adjusted to 4.2 does not work for me but ph 3.45 works, however 260/230 ratio is bad-0.5 to 0.7 is that the PH or Guanidine or alcohol that interferes?

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