Can anyone explain the FAFLP Principle?

More Leonid Ouchanov's questions See All

Buffered Growth Medium for Bacteria?

If I buffer a liquid growth medium with PBS (say I use 100 mL of PBS and 900 mL of dd H20 to dissolve the required amount of dried liquid medium) and my starting pH is 7, how effective would it be...

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Suggestions on H2O2 Detection (colorimetry)?

Hello, I am trying to design a simple assay whereby I could measure various concentrations of peroxide in an analyte. Copper sulphate gives nice color gradation upon addition of peroxide and...

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Gene Expression Analysis using R?

Hello fellow researchers, Can anyone recommend a package for R for gene expression analysis using R?

11 December 2018 2,712 7 View

Real Time PCR Analysis and Normalization?

If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...

11 December 2018 3,483 3 View

Hippurate Hydrolysis Microplate ?

Hi, I'd like to adapt hippurate hydrolysis test to microplate reader. Has anyone done this before, maybe? I assume both Na hippurate and ninhydrin must be available as powder? The question is...

06 July 2018 3,112 0 View

Buffer system for Bacterial co-Incubation?

So, I need to grow two bacteria in one medium without letting the pH go below 5.5 and without any negative effect on bacterial growth. Which buffer would be more suitable? I read on Sigma that...

06 July 2018 4,480 0 View

Published Primers not Working?

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02 March 2018 877 12 View

How do you get Rid of Nonspecific Bands in PCR?

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02 March 2018 4,561 16 View

IDT's Oligo Analizer 3.1?

Anyone used this before? I suspect one of the oligos of my primer pair forms a hairpin ? I just don't know how to read the output oligo analyzer gives. This primer is from a publication and they...

02 March 2018 8,436 1 View

Is that C. jejuni?

Can someone take a look and confirm whether what I isolated is indeed C. jejuni? I haven't got any primers yet, so I can't confirm by PCR, but the culture is gram negative, catalaze +, oxidase +....

02 March 2017 4,393 13 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

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Can thermal cycler machines be problematic during PCR?

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26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

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26 February 2021 5,029 2 View

Aly Savadogo

Dear Leonid

I recommend you this paper :http://www.microbiologyresearch.org/docserver/fulltext/jmm/50/5/mjm5005.393.pdf?expires=1516384361&id=id&accname=guest&checksum=A79266A484F646D4C257DDD312FF75C8

Sourav Maiti

It's fluorescent based AFLP. Genetic information is fragmented and polymorphism is analysed using fluorescent marker. Useful in cluster identification and strain typing. It's claimed to be cheaper than MLST. Discriminatory power is at par with MLST, MLEE and PFGE. If PFGE is not feasible, it could be tried.