IDT's Oligo Analizer 3.1?

03 March 2018 1 9K Report

Anyone used this before? I suspect one of the oligos of my primer pair forms a hairpin ? I just don't know how to read the output oligo analyzer gives.

This primer is from a publication and they supposedly successfully amplified the desired gene.

If you look at the picture it says DET next to the hairpin picture-does "det" mean "detected"?

If that's the case, what's the best way to overcome this problem -besides designing new primers, which I can't do right now.

Thank you !

Leonid Ouchanov

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