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Questions related from Leonid Ouchanov
If I buffer a liquid growth medium with PBS (say I use 100 mL of PBS and 900 mL of dd H20 to dissolve the required amount of dried liquid medium) and my starting pH is 7, how effective would it be...
05 May 2019 10,103 2 View
Hello, I am trying to design a simple assay whereby I could measure various concentrations of peroxide in an analyte. Copper sulphate gives nice color gradation upon addition of peroxide and...
03 March 2019 733 2 View
If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...
12 December 2018 3,578 3 View
Hello fellow researchers, Can anyone recommend a package for R for gene expression analysis using R?
12 December 2018 2,798 7 View
So, I need to grow two bacteria in one medium without letting the pH go below 5.5 and without any negative effect on bacterial growth. Which buffer would be more suitable? I read on Sigma that...
07 July 2018 4,578 0 View
Hi, I'd like to adapt hippurate hydrolysis test to microplate reader. Has anyone done this before, maybe? I assume both Na hippurate and ninhydrin must be available as powder? The question is...
07 July 2018 3,198 0 View
Anyone used this before? I suspect one of the oligos of my primer pair forms a hairpin ? I just don't know how to read the output oligo analyzer gives. This primer is from a publication and they...
03 March 2018 8,514 1 View
So, here is what I am dealing with: The Tm of the primers is 55 for the forward one and 56 for the reverse one. In -silico PCR produces 750 BP band, but in reality I get 3 bands. The third one...
03 March 2018 4,684 16 View
So, I have some primer pairs from a publication that work in silico, but do not give me any PCR product. I tried all annealing temperatures, upped Mg++, did trouchdown PCR-no result. Anyone has...
03 March 2018 996 12 View
Hi, It seems that this method has advantages over PFGE when it comes to distinguishing bacterial strains, however the principle of this method remains elusive. What I do not understand is the...
01 January 2018 1,860 3 View
Can someone take a look and confirm whether what I isolated is indeed C. jejuni? I haven't got any primers yet, so I can't confirm by PCR, but the culture is gram negative, catalaze +, oxidase +....
03 March 2017 4,483 13 View
Hello Members of RG Community, I am looking for a collaborator as part of my PhD project in Agricultural Sciences. My goal is to bring in a project that will require proficiency in molecular...
04 April 2016 1,062 4 View
I need to purify protein from the supernatant of bacteria grown in broth. Does anyone know what is the best way to isolate the total protein from the sup?
04 April 2016 6,330 7 View
I am trying to bind my plasmid DNA to silica columns. 4.5 M Guanidine Thiocyanate, 1 M K acetate PH adjusted to 4.2 does not work for me but ph 3.45 works, however 260/230 ratio is bad-0.5 to 0.7...
07 July 2014 7,955 2 View
