Hi,
I'd like to adapt hippurate hydrolysis test to microplate reader. Has anyone done this before, maybe? I assume both Na hippurate and ninhydrin must be available as powder? The question is what wavelength do you read the purple color? 610?
If I buffer a liquid growth medium with PBS (say I use 100 mL of PBS and 900 mL of dd H20 to dissolve the required amount of dried liquid medium) and my starting pH is 7, how effective would it be...
04 May 2019 10,168 2 View
Hello, I am trying to design a simple assay whereby I could measure various concentrations of peroxide in an analyte. Copper sulphate gives nice color gradation upon addition of peroxide and...
02 March 2019 783 2 View
Hello fellow researchers, Can anyone recommend a package for R for gene expression analysis using R?
11 December 2018 2,866 7 View
If I measure gene expression in an organism under two different conditions, do I still need to normalize these genes to a housekeeping gene or comparison between the genes under two different...
11 December 2018 3,636 3 View
So, I need to grow two bacteria in one medium without letting the pH go below 5.5 and without any negative effect on bacterial growth. Which buffer would be more suitable? I read on Sigma that...
06 July 2018 4,642 0 View
So, I have some primer pairs from a publication that work in silico, but do not give me any PCR product. I tried all annealing temperatures, upped Mg++, did trouchdown PCR-no result. Anyone has...
02 March 2018 1,074 12 View
So, here is what I am dealing with: The Tm of the primers is 55 for the forward one and 56 for the reverse one. In -silico PCR produces 750 BP band, but in reality I get 3 bands. The third one...
02 March 2018 4,732 16 View
Anyone used this before? I suspect one of the oligos of my primer pair forms a hairpin ? I just don't know how to read the output oligo analyzer gives. This primer is from a publication and they...
02 March 2018 8,558 1 View
Hi, It seems that this method has advantages over PFGE when it comes to distinguishing bacterial strains, however the principle of this method remains elusive. What I do not understand is the...
31 December 2017 1,913 3 View
Can someone take a look and confirm whether what I isolated is indeed C. jejuni? I haven't got any primers yet, so I can't confirm by PCR, but the culture is gram negative, catalaze +, oxidase +....
02 March 2017 4,546 13 View
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...
29 July 2024 1,981 1 View
Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color
28 July 2024 286 0 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
I used Aqua regia to clean Au nanoparticles in the used glassware. But for some reasons, it is not heating up upon mixing. The color did change from no color to light orange. The Au NPs on the...
25 July 2024 4,390 0 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
Hello everyone, I am currently working on a project where I aim to analyze color preferences in data visualizations across participants from three different countries. The dependent variable in...
19 July 2024 4,178 2 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View