Anyone can suggest me an alternative to the Strength and Difficulties Questionnaire (SDQ)?

Dear all, I am going to conduct a study with children and their parents. I wanted to use the Strength and Difficulties Questionnaire (SDQ), a brief behavioural and emotional screening...

06 September 2020 5,738 3 View

Is there anyone who know how to use seed function in process macro for SPSS?

Hayes (2013) is talking about using the same sample at each bootstrap analysis by setting the random number seed to the same value for each run. In this way, you can have the same confidence...

13 May 2020 5,751 5 View

Is SILAR technique can be useful for preparation of nitride thin films?

e.g. titanium nitride

26 September 2017 1,945 3 View

What is the difference between MCF-7 and A549?

26 September 2017 4,115 3 View

How to eliminate the cellular oxidative stress that is caused by a treatment?

17 April 2016 6,248 7 View

How do I crystallize ATRP?

Hello! We synthesized t-Boc-aminoethyl 2-bromoisobutyrate (white solid). Following the removal of t-Boc protection group, we are still trying desperately to crystallize the final product (i.e....

17 December 2015 3,838 2 View

How to represent control group with error bars on the relative expression histograms in a qPCR study.

I have a question regarding to the representation of the relative expression histograms. We did a comparative qPCR study, and, using the Pfaffl's efficiency corrected Ct formula, I calculated the...

11 February 2013 5,199 3 View

Looking for suggestions on a free-of-charge publishing International Astrophysics Journal with a good Impact Factor

I would like to publish my Scientific Preprint Paper free-of-charge in an international Astrophysics journal with a satisfactory Impact Factor. Can you please suggest such a journal? I have...

01 January 1970 5,991 15 View

Could new manufacturing technologies empower individuals or make them more vulnerable?

01 January 1970 4,455 3 View

Comparative Research - Case selection

I do research on environmental civil society organizations in post-Fukushima Japan. I thrive on comparative research between Japan and the US. Currently, my main focus is =to highlight the...

01 January 1970 9,005 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View