Does anyone know a suitable way or reagent to transfect MEFs cell line?

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How to solve this error messeage,Error EDDDAV: Call to ZHEGV failed. Returncode = 7 1 8?

Hi everyone, I am doing a VASP calculation, (convergence) and I encounter the error code "Error EDDDAV: Call to ZHEGV failed. Returncode = 7 1 8". I do not know how to solve it. INCAR: ALGO...

03 June 2024 9,276 2 View

What is the best software in chemical engineering in the field of thermodynamics?

field of thermodynamics?

03 January 2024 2,922 1 View

What are the steps to improve the gasoline industry?

software

31 December 2023 1,244 0 View

How to apply artificial intelligence in adsorption using Matlab ?

DATA

31 December 2023 6,582 2 View

HOW DO Estimate of the Bubble Point Pressure of Multicomponent Reservoir Hydrocarbon Fluids ?

Estimation of the Bubble Point Pressure of Multicomponent Reservoir Hydrocarbon Fluids

23 November 2023 1,859 2 View

How to apply artificial intelligence in adsorption using Matlab ?

APPLICATION OF LAB

18 November 2023 4,671 3 View

What are the best books for learning artificial intelligence for application in chemical engineering?

application of ANN

18 November 2023 3,110 0 View

Is it good to use absolute ethanol or diluted ethanol for extraction of phytochemicals?

09 March 2023 7,688 30 View

Is using 6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898) with AML cell lines possible?

Hello, I am trying to optimize the 6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898), and I have some question about it. I am confused as to why the samples become yellow....

27 February 2023 7,505 0 View

Is there anyway one can increase the protein level of Agricultural waste products.?

As most Agricultural byproducts are considered waste and they usual contain protein and others like fibre, Ash and so on, so is there anyway or means be it laboratory techniques or biotechnology...

10 January 2022 6,411 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View