Hello,
I am trying to optimize the 6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898), and I have some question about it. I am confused as to why the samples become yellow. Moreover, the Bacground signals are higher than the test. I began with 100 ug/well and gradually decreased the concentration to 5 ug/well. This implies that the samples may include an interfering chemical. Nevertheless, using 10 Kda columns did not address the problem. what are your opinions on this kit? any thought how to address this issue?
Thanks in advance for you help
Respectfully,
Abdulsalam Alruwaili