Does anyone here use chitinase, beta-glucanase and chitosanase as a preventive reagent for fungal and/or yeast in cell culture?
I found someone in reseachgate recommended this over fungizone.
In my personal experience, antifungal properties of beta-glucanases
are a way less robust than what it appears like from the literature.
Can anyone give web links and published e-books and papers telling about why anti-tumor drugs killed cancer cells first before normal cells/stem cells? Generally it must be related to metabolic...
01 February 2018 8,250 0 View
writing 10 power 4 such as 10^4 or 10e4 rather than making 4 as super script in publish papers. Can let let me have links for such papers if you have seen. Thanks.
31 December 2017 3,191 0 View
I was looking for ingredients of serum free freezing medium but I cannot find anywhere or its discussion in this research gate. So Can anyone share your knowledege on making serum free freezing...
09 October 2017 9,271 5 View
I like to know whether it is possible just to wash out trypsin with PBS, centrifuge followed by resuspending the pellet with serum free medium for cells to be cultured with serum free medium?
08 September 2017 4,327 6 View
I would like to know your experience on culturing Human breast cancer stem cells (Parental) Thanks.
07 August 2017 7,330 4 View
I would like to get some practical info with regard to biolog PMM phenotyping. I also have done some and would like to share info too.
06 July 2017 1,282 2 View
I get fold change of miRNAs expression after running qPCR. I would like to know whether I need to do statistical analysis for those fold change or not? in fold change, if expression level is above...
05 June 2017 5,018 5 View
I am currently culturing primary breast cancer epithelial cells from human breast cancer patient. It has been about 3 to 4 months and now epithelial cells are growing well and no more fibroblast...
09 October 2016 3,084 4 View
How should we look for list of journals of Q1, Q2, Q3 respectively? I cannot find any proper list. Some said the higher the impact factor, the higher the tier is and other said no, it is not as...
07 August 2016 2,529 0 View
I am facing an issue of debris after dissociation. These debris are mixed with dissociated cells. As I am using serum free and ultra low attachment flask, it is really hard to remove them. If you...
06 July 2016 8,998 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View