Can anyone give web links and published e-books and papers?

Can anyone give web links and published e-books and papers telling about why anti-tumor drugs killed cancer cells first before normal cells/stem cells? Generally it must be related to metabolic...

01 February 2018 8,093 0 View

Has any one seen writing 10 power 4 as 10^4 or 10e4 in published paper?

writing 10 power 4 such as 10^4 or 10e4 rather than making 4 as super script in publish papers. Can let let me have links for such papers if you have seen. Thanks.

31 December 2017 3,028 0 View

How to make serum free freezing medium?

09 October 2017 9,085 5 View

Instead of neutralizing trypsin by FBS, how do you think if we can just wash them out with PBS?

08 September 2017 4,154 6 View

Has anyone here experience with culturing Breast cancer stem cells (Parental)?

07 August 2017 7,179 4 View

Is any one here using Biolog PMM for mammalian cells phenotyping?

06 July 2017 1,123 2 View

Do we need to do statistical analysis for miRNA expression that showed in fold change?

I get fold change of miRNAs expression after running qPCR. I would like to know whether I need to do statistical analysis for those fold change or not? in fold change, if expression level is above...

05 June 2017 4,877 5 View

What is the best way to prove primary breast cancer epithelial cells in cultured flasks are tumor cells?

I am currently culturing primary breast cancer epithelial cells from human breast cancer patient. It has been about 3 to 4 months and now epithelial cells are growing well and no more fibroblast...

09 October 2016 2,887 4 View

What are the Q1, Q2, Q3 or Tier 1, 2, 3 journals in life science?

How should we look for list of journals of Q1, Q2, Q3 respectively? I cannot find any proper list. Some said the higher the impact factor, the higher the tier is and other said no, it is not as...

07 August 2016 2,338 0 View

What is the ideal wave length to apply for MTT assay plate reading?

I would like to add one query here about plate reading wave length. It is very confusing as some use 570 and ref 630, some other use 540 and ref 720, and also found 570 but no reference. So what...

06 July 2016 715 6 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View