Is it also effective on fungal zoospores? I'm trying to extract DNA from B. dendrobatidis Fungi and its zoospore. Can I use Nonidet P-40 to lyse fungal cell wall and zoospore?
I don't have any experience about your experiment. " using Nonidet P-40 (NP-40) to lyse fungal cell wall and zoospore" look like a new idea to prepare zoospore. I think you to do some test. 1.use solution containing different concentration of NP-40 to test if NP-40 can lyse fungal cell wall, you go to 2. to optimizing condition for lyse fungal cell wall, and analysis zoospore.