Why Maximum Parsimony in PAUP 4.0 does not work for me?

07 August 2016 3,504 0 View

Why I get weird curves in qPCR?

some times my standard DNA also gives me weird curves..... I use taqman probes for standard curve analysis... following are some pics attached showing result of qPCR run. Can anybody help me with...

09 October 2015 7,689 8 View

What is a size of ITS region of Fungus??

?

07 August 2015 2,636 0 View

Can we use regular PCR master mix for qPCR assay?

Can we use regular PCR master mix for qPCR assay? Or we should buy qPCR master mix. I am going to use taqman probe for my qPCR assay.

03 April 2015 6,277 10 View

How to deal with with PCR inhibitors?

how to deal with PCR inhibitors ? what should we add or do to remove PCR inhibitors...

11 December 2014 7,801 7 View

What is the the minimum percentage of identity required by BLAST to tentatively determine new species of bacteria?

09 October 2014 3,833 33 View

What is the minimum DNA quantity (ng) required to visualize DNA on Agarose gel?

08 September 2014 2,314 40 View

Can anybody tell me which is the best DNA extraction method for total DNA extraction from cotton swabs?

08 September 2014 1,572 7 View

How many pUC18 plasmid DNA molecules will be there in 10 ng/ul conc.?

I would like know how many pUC 18 molecules will be there in 10 ng/ul concentration.. can anyone tell me how to calculate it.???

08 September 2014 3,663 4 View

Does anyone have used Nonidet P-40 for Fungal DNA extraction?

Is it also effective on fungal zoospores? I'm trying to extract DNA from B. dendrobatidis Fungi and its zoospore. Can I use Nonidet P-40 to lyse fungal cell wall and zoospore?

08 September 2014 455 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View