I am trying to generate MP tree in PAUP 4.0. When I tried to give following command , It does not go accordingly. The addseq will be always 1 during the run. So why this is happening?? any Idea???
Hsearch addseq=random nreps=1000
some times my standard DNA also gives me weird curves..... I use taqman probes for standard curve analysis... following are some pics attached showing result of qPCR run. Can anybody help me with...
09 October 2015 7,858 8 View
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07 August 2015 2,743 0 View
Can we use regular PCR master mix for qPCR assay? Or we should buy qPCR master mix. I am going to use taqman probe for my qPCR assay.
03 April 2015 6,410 10 View
how to deal with PCR inhibitors ? what should we add or do to remove PCR inhibitors...
11 December 2014 7,904 7 View
I have a 16s rRNA sequence which is showing 96% identity, so it might be a new species?
09 October 2014 4,111 33 View
I have very low amount of DNA 10 ng/ul. So if I would like to check on gel, how much ul I have to take?
08 September 2014 2,715 40 View
I'm trying to extract Bd fungal DNA from a cotton swabs after swabbing on frogs. I'm trying to get total DNA, but I'm not getting it. Right now I'm using lysis buffer containing EDTA, Tris HCl,...
08 September 2014 1,670 7 View
I would like know how many pUC 18 molecules will be there in 10 ng/ul concentration.. can anyone tell me how to calculate it.???
08 September 2014 3,792 4 View
I'm working on a disease causing fungus which only infects amphibians and I would like know which genes from this fungus are responsible for disease. So to do this, how can I proceed? Any method...
08 September 2014 5,863 3 View
Is it also effective on fungal zoospores? I'm trying to extract DNA from B. dendrobatidis Fungi and its zoospore. Can I use Nonidet P-40 to lyse fungal cell wall and zoospore?
08 September 2014 573 1 View
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Hello experts, Does anyone know any free software about retention index prediction ?
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After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
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I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
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In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
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