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I am trying to generate MP tree in PAUP 4.0. When I tried to give following command , It does not go accordingly. The addseq will be always 1 during the run. So why this is happening?? any...
07 August 2016 3,607 0 View
some times my standard DNA also gives me weird curves..... I use taqman probes for standard curve analysis... following are some pics attached showing result of qPCR run. Can anybody help me with...
09 October 2015 7,858 8 View
Can we use regular PCR master mix for qPCR assay? Or we should buy qPCR master mix. I am going to use taqman probe for my qPCR assay.
03 April 2015 6,410 10 View
how to deal with PCR inhibitors ? what should we add or do to remove PCR inhibitors...
11 December 2014 7,904 7 View
I have a 16s rRNA sequence which is showing 96% identity, so it might be a new species?
09 October 2014 4,111 33 View
I have very low amount of DNA 10 ng/ul. So if I would like to check on gel, how much ul I have to take?
08 September 2014 2,715 40 View
I'm trying to extract Bd fungal DNA from a cotton swabs after swabbing on frogs. I'm trying to get total DNA, but I'm not getting it. Right now I'm using lysis buffer containing EDTA, Tris HCl,...
08 September 2014 1,670 7 View
I would like know how many pUC 18 molecules will be there in 10 ng/ul concentration.. can anyone tell me how to calculate it.???
08 September 2014 3,792 4 View
I'm working on a disease causing fungus which only infects amphibians and I would like know which genes from this fungus are responsible for disease. So to do this, how can I proceed? Any method...
08 September 2014 5,863 3 View
Is it also effective on fungal zoospores? I'm trying to extract DNA from B. dendrobatidis Fungi and its zoospore. Can I use Nonidet P-40 to lyse fungal cell wall and zoospore?
08 September 2014 573 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
