Does anyone have tips or tricks for isolation and assessment of Regulatory T cells from umbilical cord blood?

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Zhan he Wu

From my experience, the magnetic bead isolation is a good technique to isolate T cells from cord.

Jennifer Gilner

Have you done the isolation from frozen mononuclear cell fraction, or only from fresh cord? If you have done from frozen, is there a significant loss of yield?

Only on fresh cells not frozen cells. I believe antigens on cell surface need to be recovered after frozen. Give a try