Or can someone recommend another DCF-DA to use for a flow? I would like to measure ROS after treating the cells with H2O2, like to buy the right DCF-DA, any suggestion will be good.
Hi Daisy.....we have been using CM-H2DCF-DA from Life Technologies-Invitrogen since very long....working perfectly well......My recent article deals with ROS....You can check it there....
I agree with all, I started using the Invitrogen probe with MACSQuant, also with BD scan. Depending on the machine, you will have to play with voltages (as usual) for optimization. Good luck with your research!