Does anyone know the list of genes that regulates calcium ion channel?

More Daisy Mohanasundaram's questions See All

Does anyone have experience wtih the DCF-DA from invitrogen lifetec product for ROS measurement by flow?

Or can someone recommend another DCF-DA to use for a flow? I would like to measure ROS after treating the cells with H2O2, like to buy the right DCF-DA, any suggestion will be good.

06 July 2013 5,747 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Geneviève Bart

There are thousands of ion channels, they also have accessory sub-unit some of which are regulatory or sensing.

In addition there are calcium-pump which are usually activated when signal triggers calcium release from the internal stores, and calcium exchanger (Ca/Na, etc..) and store refilling Ca channel and accessories like ORAI1,... et STIM1,...

Protein kinase activated by calcium like CAMKII (Which have Ca-regulated modulator like PCP4 or related), classical PKCs, et many others which also control ion channels (PKG, PKA, PKD, etc..... )

Your question needs more focus to be answered, what type of molecules or pathways are you interested in.

Daisy Mohanasundaram

Hi Bart,

Thank you for responding to my question. I am interested in membrane bound calcium efflux receptor or gene that enhances the calcium flux from and to extacellular matrix to intracellular.