Heba s Dosoki

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How could I measure the extracellular and intracellular H2O2 in stimulated and unstimulated cells?

Does anyone have experience with ROS generation assay (Homovanilic acid)? Has anybody used this type of assay? Could anybody provide me with successful protocol for that? Is it reliable and specific?

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What exactly happens during the 24 hours post-transfection?

My protocol is to transfect the fibroblasts for 24hrs and then directly in the next day starvation for further 24hrs and then stimulate with a growth factor. My question is: would it be better to...

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