How long after Critical Point Drying (CPD) step, samples still good for SEM?

Anyone has experience about this? Thank in advance.

09 October 2016 2,625 0 View

Fix OsO4 for SEM preparation sample?

06 July 2016 8,658 4 View

Measurement accurate amount of components in rice seed (starch, protein and lipid)?

Do anyone can suggest me the effective method to analysis components in rice seed, especially starch? Thank you in advance.

06 July 2016 6,615 6 View

Is anyone familiar with Indica and Japonica flag leaf?

03 April 2015 6,889 2 View

How do I determine the transcription start site?

I am working on the gene which has DNA sequence. However, database do not support full length cDNA (just a part of it can be identified and database announce that ATG can not be detected). How can...

02 March 2015 6,893 7 View

Can anybody help me to design a primer for cloning?

09 October 2014 8,727 5 View

Can anyone tell the protocol for stomatal movement in rice?

Please support more details

06 July 2014 2,755 0 View

Can anyone help with concentration of jasmonic acid/methyl jasmonate in vitro?

For hormone treatment in short term (around 6-12 hours), 100uM jasmonic acid/methyl jasmonate is enough to induce plant. In case in a long term (7-10 days), which concentration is suitable for rice?

04 May 2014 9,337 1 View

How can I detect a phenotype?

I have found dwarf phenotype, cosegregate to T-DNA insertion. My problem is: 1. T_DNA insertion (pGA2715 vector) locates between 2 genes(A and B), upstream of A around 82bp, expression level of A...

03 April 2014 309 4 View

Anyone have experience or tips regarding RNAi cloning?

I cloned an RNAi construct. First, I amplified the 250bp fragment and the inserted into pBluescript II SK. pBluescript II SK was cut by SmaI. But when I grew on the media with ampicillin, all...

01 February 2014 573 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View