Fix OsO4 for SEM preparation sample?

06 July 2016 8,658 4 View

Measurement accurate amount of components in rice seed (starch, protein and lipid)?

Do anyone can suggest me the effective method to analysis components in rice seed, especially starch? Thank you in advance.

06 July 2016 6,615 6 View

Is anyone familiar with Indica and Japonica flag leaf?

03 April 2015 6,889 2 View

How do I determine the transcription start site?

I am working on the gene which has DNA sequence. However, database do not support full length cDNA (just a part of it can be identified and database announce that ATG can not be detected). How can...

02 March 2015 6,893 7 View

Can anybody help me to design a primer for cloning?

09 October 2014 8,727 5 View

Does anyone have experience with sampling pollen?

07 August 2014 3,560 2 View

Can anyone tell the protocol for stomatal movement in rice?

Please support more details

06 July 2014 2,755 0 View

Can anyone help with concentration of jasmonic acid/methyl jasmonate in vitro?

For hormone treatment in short term (around 6-12 hours), 100uM jasmonic acid/methyl jasmonate is enough to induce plant. In case in a long term (7-10 days), which concentration is suitable for rice?

04 May 2014 9,337 1 View

How can I detect a phenotype?

I have found dwarf phenotype, cosegregate to T-DNA insertion. My problem is: 1. T_DNA insertion (pGA2715 vector) locates between 2 genes(A and B), upstream of A around 82bp, expression level of A...

03 April 2014 309 4 View

Anyone have experience or tips regarding RNAi cloning?

I cloned an RNAi construct. First, I amplified the 250bp fragment and the inserted into pBluescript II SK. pBluescript II SK was cut by SmaI. But when I grew on the media with ampicillin, all...

01 February 2014 573 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View