Anyone has experience about this?
Thank in advance.
Preparation sample for SAM, samples were fixed with glutaraldehyde and then post-fixed with OsO4. OsO4 is extremely toxic to human and damage proteins and other components of sample. Could I skip...
06 July 2016 8,771 4 View
Do anyone can suggest me the effective method to analysis components in rice seed, especially starch? Thank you in advance.
06 July 2016 6,816 6 View
Does anyone have any information on the different sizes of Indica and Japonica flag leaves? Thank you in advance.
03 April 2015 6,999 2 View
I am working on the gene which has DNA sequence. However, database do not support full length cDNA (just a part of it can be identified and database announce that ATG can not be detected). How can...
02 March 2015 7,007 7 View
I am having trouble with designing a primer for cloning Y2H. My full length RNA sequence from source lacks 5'-UTR region, it starts at ATG, then I must design a primer from ATG. The trouble is...
09 October 2014 8,808 5 View
How can sampling rice pollen at unicellular, bicellular and tricellular for RNA extraction? Anyone has experience about this work, please help.
07 August 2014 3,628 2 View
Please support more details
06 July 2014 2,958 0 View
For hormone treatment in short term (around 6-12 hours), 100uM jasmonic acid/methyl jasmonate is enough to induce plant. In case in a long term (7-10 days), which concentration is suitable for rice?
04 May 2014 9,411 1 View
I have found dwarf phenotype, cosegregate to T-DNA insertion. My problem is: 1. T_DNA insertion (pGA2715 vector) locates between 2 genes(A and B), upstream of A around 82bp, expression level of A...
03 April 2014 383 4 View
I cloned an RNAi construct. First, I amplified the 250bp fragment and the inserted into pBluescript II SK. pBluescript II SK was cut by SmaI. But when I grew on the media with ampicillin, all...
01 February 2014 657 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...
09 August 2024 7,892 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
to identify themes in question with APA style references
07 August 2024 2,239 5 View
Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...
06 August 2024 9,986 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
