21 Questions 25 Answers 0 Followers
Questions related from Tuyet Van
Anyone has experience about this? Thank in advance.
10 October 2016 2,669 0 View
Do anyone can suggest me the effective method to analysis components in rice seed, especially starch? Thank you in advance.
07 July 2016 6,662 6 View
Preparation sample for SAM, samples were fixed with glutaraldehyde and then post-fixed with OsO4. OsO4 is extremely toxic to human and damage proteins and other components of sample. Could I skip...
07 July 2016 8,739 4 View
Does anyone have any information on the different sizes of Indica and Japonica flag leaves? Thank you in advance.
04 April 2015 6,952 2 View
I am working on the gene which has DNA sequence. However, database do not support full length cDNA (just a part of it can be identified and database announce that ATG can not be detected). How can...
03 March 2015 6,939 7 View
I am having trouble with designing a primer for cloning Y2H. My full length RNA sequence from source lacks 5'-UTR region, it starts at ATG, then I must design a primer from ATG. The trouble is...
10 October 2014 8,774 5 View
How can sampling rice pollen at unicellular, bicellular and tricellular for RNA extraction? Anyone has experience about this work, please help.
08 August 2014 3,600 2 View
Please support more details
07 July 2014 2,796 0 View
For hormone treatment in short term (around 6-12 hours), 100uM jasmonic acid/methyl jasmonate is enough to induce plant. In case in a long term (7-10 days), which concentration is suitable for rice?
05 May 2014 9,379 1 View
I have found dwarf phenotype, cosegregate to T-DNA insertion. My problem is: 1. T_DNA insertion (pGA2715 vector) locates between 2 genes(A and B), upstream of A around 82bp, expression level of A...
04 April 2014 352 4 View
It must be high concentration of primer for iPCR or tail-PCR, but that's too high, almost 1.000.000 times higher than usual. How concentration can be used for iPCR or tail-PCR, it can be around...
02 February 2014 8,011 6 View
I cloned an RNAi construct. First, I amplified the 250bp fragment and the inserted into pBluescript II SK. pBluescript II SK was cut by SmaI. But when I grew on the media with ampicillin, all...
02 February 2014 622 3 View
I would like to make the promoter comparison of one gene between Japonica and Indica cultivar.
01 January 2014 1,896 9 View
.
12 December 2013 5,822 2 View
12 December 2013 9,457 1 View
I have plant to cut plasmid using double digestion (SmaI and KpnI). These two enzymes are different about the condition. The suggestion is sequential digestion. Any advice?
12 December 2013 478 2 View
I'm looking for the gene which can be a positive control under drought conditions for real time PCR in rice. I found some papers about drought stress in rice but most of them do not use a positive...
05 May 2013 1,128 3 View
I have intensity database of gene expression, then I made log2 of them all. By the way, I checked which genes show dominant expression on which tissue, I took divided log2 intensity of each tissue...
10 October 2012 2,003 0 View
I used Ubiquitin 5 as control primer to check balance cDNA and I got a good result. But when I tried to check with another control primer like Ubiquitin 1 or OsActin, those bands were different...
03 March 2012 7,309 3 View
Actually, I can come to every reference to figure out that thing but...we have anyway else for speeding up...???
03 March 2012 7,468 8 View
I am in big trouble with ligation. I purified DNA from agarose gel with QIAquick Gel Extraction kit, I processed a tailing DNA fragment and then ligation with pGEM-T easy vector. I got blue...
02 February 2012 2,861 5 View
