Is anyone familiar with Indica and Japonica flag leaf?

More Tuyet Van's questions See All

How long after Critical Point Drying (CPD) step, samples still good for SEM?

Anyone has experience about this? Thank in advance.

09 October 2016 2,738 0 View

Fix OsO4 for SEM preparation sample?

Preparation sample for SAM, samples were fixed with glutaraldehyde and then post-fixed with OsO4. OsO4 is extremely toxic to human and damage proteins and other components of sample. Could I skip...

06 July 2016 8,771 4 View

Measurement accurate amount of components in rice seed (starch, protein and lipid)?

Do anyone can suggest me the effective method to analysis components in rice seed, especially starch? Thank you in advance.

06 July 2016 6,816 6 View

How do I determine the transcription start site?

I am working on the gene which has DNA sequence. However, database do not support full length cDNA (just a part of it can be identified and database announce that ATG can not be detected). How can...

02 March 2015 7,007 7 View

Can anybody help me to design a primer for cloning?

I am having trouble with designing a primer for cloning Y2H. My full length RNA sequence from source lacks 5'-UTR region, it starts at ATG, then I must design a primer from ATG. The trouble is...

09 October 2014 8,808 5 View

Does anyone have experience with sampling pollen?

How can sampling rice pollen at unicellular, bicellular and tricellular for RNA extraction? Anyone has experience about this work, please help.

07 August 2014 3,628 2 View

Can anyone tell the protocol for stomatal movement in rice?

Please support more details

06 July 2014 2,958 0 View

Can anyone help with concentration of jasmonic acid/methyl jasmonate in vitro?

For hormone treatment in short term (around 6-12 hours), 100uM jasmonic acid/methyl jasmonate is enough to induce plant. In case in a long term (7-10 days), which concentration is suitable for rice?

04 May 2014 9,411 1 View

How can I detect a phenotype?

I have found dwarf phenotype, cosegregate to T-DNA insertion. My problem is: 1. T_DNA insertion (pGA2715 vector) locates between 2 genes(A and B), upstream of A around 82bp, expression level of A...

03 April 2014 383 4 View

Anyone have experience or tips regarding RNAi cloning?

I cloned an RNAi construct. First, I amplified the 250bp fragment and the inserted into pBluescript II SK. pBluescript II SK was cut by SmaI. But when I grew on the media with ampicillin, all...

01 February 2014 657 3 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

Given the current level of natural phenomena cause by the climate change and environmental pollution, will the AI find the technological solutions?

AI-driven technologies offer previously unheard-of capabilities to process enormous volumes of data, extract insightful knowledge and improve predictive models, according to the UN’s World...

31 July 2024 9,733 3 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

Why microbes are used in environmental engineering and role of microbes in reducing environmental pollution and improving environmental quality?

25 July 2024 5,192 5 View

Can I separate xylene from paraffin after deparaffinization of microtome samples and reuse the xylene?

...

24 July 2024 1,559 1 View