I'm wondering if there would be a way to join 2 PCR fragments together
Probably won't work with Overlap PCR, and here's why:
I want to see if there would be any effect by methylation of a gene region.
But we know that commercially available Methyltransferases (e.g. from NEB) will methylate every CpG site in the region.
So I'm wondering if there's a way to connected in-vitro methylated PCR products (so that will be PCR amplification followed by methylation) to normal PCR products
Any insights?
Thank you very much!