Gene synthesis - FAQS.TIPS

More Donghyun Dave Lee's questions See All

ChIP on suspension cells?

Is there any difference in performing ChIP on suspension cells vs. adherent cells? I'm currently using Activemotif ChIP-IT Express Enzymatic Kit

06 July 2016 5,756 4 View

Post-midiprep plasmid sequencing?

I've successfully cloned my fragments into pGL3 (Luciferase) vector, and I want to see if the bands that I see on my agarose gel are correct by sequencing. So I was just wondering what the ideal...

10 November 2013 2,654 0 View

PCR amplification of bisulfite converted DNA?

I'm trying to amplify using bisulfite converted dna template (to preserve methylation signature) following is my target region: (and i assume that cytosines that are non-cpg are converted to t...

07 August 2013 2,465 26 View

Does anyone have experience with joining 2 PCR products together?

I'm wondering if there would be a way to join 2 PCR fragments together Probably won't work with Overlap PCR, and here's why: I want to see if there would be any effect by methylation of a gene...

06 July 2013 3,993 2 View

Cloning Basics & pGL3-basic usage

If possible, I would like some explanation about luciferase assay I'm trying to have several different portions of a specific gene promoter cloned into separate luciferase reporter vector...

06 July 2013 6,580 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Noah Abramson

You should check out Genome Compiler. You can edit any gene sequence then order it with a click of a button. You also can choose from a variety of providers (some better than others) and get them shipped right to your door. Their user interface is also super nice and easy to use: very intuitive.

Ilaria De Vito

GENEWIZ is very good for doing gene synthesis. Placing the order online is very simple and the company's team of scientists is very supportive for troubleshooting.